GENETIC ENGINERRING AND TISSUE CULTURE ASSIGNMENT

1	[A]: Biotechnology is multidisciplinary subject [R]: Biotechnology is based on the principles of only Microbiology Both A and R are true and R is the correct explanation of A Both A and R are true but R is not the correct explanation of A A is true but R is false A is false but R is true Ans:A is true but R is false
2	Choose the correct statement Biotechnology is new science to man Penicillin was discovered during world war I Acetone and Butonol are produced by a species of Clostridium Lactobacillus is used in bread making Ans:Acetone and Butonol are produced by a species of Clostridium
3	Maximum benefits of Biotechnology have been utilized by the following field Medicine Agriculture Industry Food science Ans:Medicine
4	Vitamins that are commercially produced by using microbes are I. Vitamin B12 II. Riboflavin III. Thiamine IV. Vitamin A All except II All All except III All except IV Ans:All except III
5	Large scale production of Mushrooms play a role in Bioremediation Curing nutritionally influenced diseases Industrial production of enzymes Gene therapy Ans:Bioremediation
6	Choose the incorrect pair Cytoplasmic polyhedrosis virus - Baculovirus Nutritionally influenced disease - Cancer Intergeneric hybrid plant – Protoplast fusion Bread making - Chloromycetin Ans:Bread making - Chloromycetin
7	Enzyme commonly used in used in dairy industry is Protease Rennet Amylase α-amylase Ans:Rennet
8	Study the following lists and choose the correct match List – I List – II A) Interferon I) Insecticide B) Probe DNA II) Taipei C) Golden Rice III) Sensitive biological detector D) Baculo virus IV) Viral vaccine V) Immuno modulators A - V, B - IV, C - III, D - I A - IV, B - III, C - II, D - V A - V, B - III, C - I, D - II A - V, B - III, C - II, D -I Ans:A - V, B - III, C - II, D - I
9	Study the following table and choose the correct combination II and III I and II III and IV II and IV Ans:II and III
10	Study the following table and choose the correct combination I and III II and IV I and II III and IV Ans:I and III
11	Number of genes involved in Vitamin A production is 4 3 5 2 Ans:3
12	Labeled single stranded DNA is called as C-DNA Satellite DNA Probe DNA Phasmid Ans:Probe DNA
13	[A]: Genetic engineering is based on the principle of crossing over [R]: Endonucleases and ligases are used in rDNA technology Both A and R are true and R is the correct explanation of A Both A and R are true but R is not the correct explanation of A A is true but R is false A is false but R is true Ans:Both A and R are true and R is the correct explanation of A
14	Choose the correct statement with respect to Genetic engineering The genetically modified organism has both desirable and undesirable characters of the donor Recombinants show the expression of only the donor genes but not the host genes Genetically modified organisms do not have any undesirable genes of donor organism Complete genome of the donor is inserted into the genome of host organism Ans:Genetically modified organisms do not have any undesirable genes of donor organism
15	In Vitro gene cloning is carried out by using Themister Thermocycler Thermo couple psychrometer Thermopsychrometer Ans:Thermocycler
16	If the half of the sequence of nitrogen bases of a restriction site on one strand is “TCA”, the remaining half in continuation must be TCA TGA AGT ACT Ans:TGA
17	Ezymes used during isolation of a plant gene belong to the class Oxidoreductases Transferases Hydrolases Lyases Ans:Hydrolases
18	The following enzyme is not used for separation of a desired gene from the donor organism Nuclease Cellulase Protease Ligase Ans:Ligase
19	Chilled ethanol is used in genetic engineering for Gradient centrifugation Detergent lysis of cell Precipitating DNA Gel electrophoresis Ans:Precipitating DNA
20	The sequence of nitrogen bases (Nucleotides) of a DNA strand at its restriction site is identified from 5'→ 3' as “ 1G2C3T4A”. With respect to this match the following and identify the correct match. A - III, B - V, C - II, D - IV A - IV, B - III, C - II, D - I A - III, B - IV, C - I, D - II A - III, B - V, C - II, D - I Ans:A - III, B - IV, C - I, D - II
21	[A]: DNA polymerase is used in Thermocycler [R]: Themocycler is used for in vitro synthesis of several copies of vector DNA Both A and R are true and R is the correct explanation of A Both A and R are true but R is not the correct explanation of A A is true but R is false A is false but R is true Ans:A is true but R is false
22	Arrange the usage of the following in correct sequence during isolation of gene from the donor organism I. Detergents II. Chilled ethanol III. Ultracentrifuge IV. Gel Electrophoresis I, III, II, IV III, I, II, IV I, II, IV, III II, I, III, IV Ans:I, III, II, IV
23	Lysenco coined the term Vernalisation Photoperiodism Vernalin Critical day length Ans:Vernalisation
24	Plant A can flower if exposed to a light period of 2 hours or 14 hours in a day but can not flower if not exposed continuously for a dark period of 10 hours. Plant B can not flower if not exposed continuously for a photoperiod of 10 hours and can flower if continuously exposed to 2 or 12 hours of dark period. Choose the correct statement from the following. A and B are Short day plants A is Short day plant and B is Long day plant A and B are Long day plants A is Long day plant and B is short day plant Ans:A is Short day plant and B is Long day plant
25	To isolate a gene from the donor genome by using EcoRI, the total number of hydrogen bonds and covalent bonds to be broken are respectively 16 and 4 16 and 2 8 and 4 6 and 24 Ans:16 and 4
26	Circular plasmid can be made linear by the action of Ligase Lysozyme RNA nuclease Restriction endonuclese Ans:Restriction endonuclese
27	[A]: A plasmid with several restriction sites for a restriction enzyme can not be used as vector [R]: A vector with several similar restriction sites may loose some of the useful regions needed for effective gene transfer into the host. Both A and R are true and R is the correct explanation of A Both A and R are true but R is not the correct explanation of A A is true but R is false A is false but R is true Ans:Both A and R are true and R is the correct explanation of A
28	Peptidoglycon of bacterial cell is not digested by I. EDTA II. Lysozyme III. Sodium Lauryl Sulphate I and II II and III I and III III only Ans:I and III
29	Chimeric DNA consists of Only vector DNA Only Donor DNA Vector DNA and host DNA Vector DNA and donor DNA Ans:Vector DNA and donor DNA
30	Arrange the action of the following substances in correct sequence during the method of genetic engineering. I. Sodium Lauryl Sulphate II. EDTA III. Calcium Chloride IV. Lysozyme IV, II, I, III II, IV, I, III II, IV, III,I II, I, III, IV Ans:II, IV, I, III
31	[A]: rDNA can replicate itself in the host bacterial Cell [R]: rDNA has promoter regions in the vector DNA portions as well as in the donor DNA region Both A and R are true and R is the correct explanation of A Both A and R are true but R is not the correct explanation of A A is true but R is false A is false but R is true Ans:Both A and R are true but R is not the correct explanation of A
32	During the method of genetic engineering, Antibiotics are used in one of the following steps isolation of gene in the formation of rDNA introducing the rDNA into host cell Selection of desired transformed cells Ans:Selection of desired transformed cells
33	Genetic probes are used during Reconstruction of plasmid insertion of gene into vector Inserting rDNA into host cell Colony hybridisation Ans:Colony hybridisation
34	Humulin is produced by Man normal E. coli Transgenic E. coli Phage vectors Ans:Transgenic E. coli
35	The techniques used in Plant Transgenics are I. Tissue culture II. rDNA technology III. Hybridoma I and II II and III I and III only II Ans:I and II
36	For gene transfer into host cells, the most common method used in Plant genetic engineering is Microinjection Electroporation Phage mediated gene transfer Plasmid mediated gene transfer Ans:Plasmid mediated gene transfer
37	The total number of Plant species in which Transgenic plants are produced is 20 40 30 more than 50 Ans:more than 50
38	Plasmid of the following bacterium is extensively used in Plant genetic engineering. E.coli Streptomyces Agrobacterium Erwinia Ans:Agrobacterium
39	First produced transgenic plant was a Monocot Dicot Gymnosperm Pteridophyte Ans:Dicot
40	Usage of transgenic plants for large scale production of useful products is called as Plant forming Molecular forming Plant Pharming Molecular forming Ans:Molecular forming
41	Basmati variety of rice is resistant to I. Virus II. Bacteria III. Cold IV. Salt I and II I and III II and IV III and IV Ans:III and IV
42	Choose the correct statement Insulin can not be produced by Tansgenic plants Molecular farming can revolutionise biochemical industry Genetically modified crops have evolved as alternative resources to Paper industry Male sterile Papaya can be used hybrid seed production Ans:Molecular farming can revolutionise biochemical industry
43	Flavr Savr is transgenic Potato Tomato Papaya Cotton Ans:Tomato
44	Match the following A - I, B - II, C - III, D - V A - I, B - II, C - III, D - IV A - II, B - III, C - IV, D - V A - I, B - V, C - II, D - III Ans:A - I, B - II, C - III, D - IV
45	Study the following table and choose the correct combination I and II III and IV I and III II and IV Ans:I and II
46	[A]: Transgenic plants are more efficient than plants produced by hybridization [R]: Desirable gene present in any organism can be successfully inserted into plants through genetic engineering Both A and R are true and R is the correct explanation of A Both A and R are true but R is not the correct explanation of A A is true but R is false A is false but R is true Ans:Both A and R are true and R is the correct explanation of A
47	Blindness in children can be prevented by using the following food Transgenic Taipei rice Tansgenic tomato Transgenic Potato Transgenic Papaya Ans:Transgenic Taipei rice
48	[A]: GM crops may pose threat to biodiversity [R]: Gm crops are not naturally evolved. Both A and R are true and R is the correct explanation of A Both A and R are true but R is not the correct explanation of A A is true but R is false A is false but R is true Ans:Both A and R are true and R is the correct explanation of A
49	This has application in forensic science Gene therapy Gene replacement therapy DNA finger printing Ti Plasmid Ans:DNA finger printing
50	The environmental concern of genetically modified crops is/are I. Development of herbicidal resistance in weeds II. Transfer of allergens to domestic animals III. Alteration of normal evolutionary pattern IV. Change in fundamental nature of vegetables. I and II III and IV I and III II and IV Ans:I and III
51	Field trials of genetically modified crops are monitored by Department of Biotechnology IBCS GEAC RCGM Ans:GEAC
52	The ultimate molecular switches are Plasmids Restriction sites Host cells Genes Ans:Genes
53	Genetically engineered microorganisms are used in Gene therapy Gene replacement therapy DNA fingerprinting Environmental clean up Ans:Environmental clean up
54	[A]: Some transgenic plants are not beneficial to mankind. [R]: Products of transgenic plants may posses allergens Both A and R are true and R is the correct explanation of A Both A and R are true but R is not the correct explanation of A A is true but R is false A is false but R is true Ans:Both A and R are true and R is the correct explanation of A
55	Various diseases of human beings are easily diagnosed by DNA finger printing Hybridoma technology rDNA technology using Microbes Ans:rDNA technology
56	Match the following with respect to Genetic engineering A - V, B - III, C - IV, D - II A - IV, B - V, C - III, D - I A - V, B - III, C - IV, D - I A - V, B - IV, C - II, D - III Ans:A - V, B - III, C - IV, D - I
57	Tissue culture is a major biotechnological tool in I. Agriculture II. Industry III. Horticulture IV. Forestry I and III All except II All except IV All Ans:All
58	[A]: Sieve cells are not totipotent [R]: They do not have mitochondria Both A and R are true and R is the correct explanation of A Both A and R are true but R is not the correct explanation of A A is true but R is false A is false but R is true Ans:A is true but R is false
59	Totipotency in plants was first experimentally shown by Steward Morgan Muller White Ans:Steward
60	The first plant developed through tissue culture has the following morphological characters I. Involucel II. Storage roots III. Pedicellate flowers IV. Didynamous androecium I, II, III II, III, IV I, II, IV I, II, III, IV Ans:I, II, III
61	Basal medium is devoid of Vitamins Amino acids Kinetin Sugars Ans:Kinetin
62	The following of Culture medium is not a nutrient Agar Amino acids Sugar Water Ans:Agar
63	Arrange the usage of the following in correct sequence during the method of tissue culture I. Cotton Plugs II. pH strips III. Organic and inorganic nutrients IV. Agar III, IV, II, I III, II, IV, I II, III, IV, I III, II, I, IV Ans:III, II, IV, I
64	The pH of the nutrient medium must be maintained between 5.8 - 6.0 5.6 - 6.6 6.0 – 6.4 5.6 – 6.0 Ans:5.6 – 6.0
65	The following conditions have to maintained during autoclaving 121 °C temp. 15 lb pressure, 15 min. time 115°C temp. 15 lb pressure, 15 min. time 151°C temp. 12 lb pressure, 15 min. time 121°C temp. 15 lb pressure, 12 min. time Ans:121°C temp. 15 lb pressure, 15 min. time
66	[A]: Both Sodium hypochlorite and 0.1% Mercuric chloride are used during the preparation of explant selected from a younger leaf. [R]: Both are surface sterilizers. Both A and R are true and R is the correct explanation of A Both A and R are true but R is not the correct explanation of A A is true but R is false A is false but R is true Ans:A is false but R is true
67	Autoclaved distilled water must be used during the following stage of Tissue Culture. Preparation of Explant Preparation of Nutrient medium Addition of Agar-Agar Sterilization of Culture vials Ans:Preparation of Explant
68	Benzyl amine is used in tissue culture for I. Rhizogeness II. Caulogenesis III. Obtaining seedlings from seeds only I II and III I and II Only II Ans:I and II
69	[A]: 2,4-D is preferred over IAA in tissue culture [R]: 2,4-D is not destroyed by light and high temperature. Both A and R are true and R is the correct explanation of A Both A and R are true but R is not the correct explanation of A A is true but R is false A is false but R is true Ans:Both A and R are true and R is the correct explanation of A
70	The culture vials with nutrient medium must be plugged with non-absorbent cotton because It prevents the entry of microbes and gases promotes the entry of gases but not exit of gases It prevents the entry of microbes and promotes exchange of gases it prevents the leakage of nutritent medium Ans:It prevents the entry of microbes and promotes exchange of gases
71	The following one should not be selected as explant during tissue culture Hypodermis of dicot stem Hypodermis of Cycas leaflet Abaxial epidermis of Maize leaf Adaxial epidermis of Sunflower leaf Ans:Hypodermis of Cycas leaflet
72	Explant is Plant developed in previous experiment of tissue culture A living part of plant introduced to culture medium complete seedling obtained from seeds upon growing on nutrient medium Plant formed through tissue culture Ans:A living part of plant introduced to culture medium
73	Most popular culture medium used in tissue culture was prepared by Murashige and Steward medium Maheswari and Skoog medium Murashige and Skoog medium Miller and Skoog medium Ans:Murashige and Skoog medium
74	In Tissue culture Sodium alginate is used for Preparation of Explant Making nutrient medium solid Making synthetic seed Preparation of Soil rite Ans:Making synthetic seed
75	Formation of the following is usually a must during the method of tissue culture Callus Somatic embryos Plantlets Embryoids Ans:Plantlets